Part:BBa_K2984036

L1c-PsaD-PETase-YFP-RBCS2

This part is composed of the PsaD promoter (BBa_K2984008), a B2-B2 linker (BBa_K2984034), the PETase enzyme (), a YFP mVenus marker (BBa_K2984024), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression of the PETase.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1915
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1244
  Illegal PstI site found at 1915
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4
  Illegal XhoI site found at 968
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1915
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1915
  Illegal NgoMIV site found at 2392
  
• 1000
  COMPATIBLE WITH RFC[1000]