Primer

Part:BBa_K2982007:Design

Designed by: Tsang Hoi Yeung, Ocean   Group: iGEM19_HK_GTC   (2019-10-15)
Revision as of 12:46, 16 October 2019 by T ocean (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Forward primer with BBa_J61124 for amplifying BBa_E0040


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has a XbaI restriction site that allows the cutting of the part. It also contains linkage sequences of the PET-28a vector between the RE site and RBS, also between RBS and the GFP gene.

Source

iGEM registry

References