Primer

Part:BBa_K2982008

Designed by: Tsang Hoi Yeung, Ocean   Group: iGEM19_HK_GTC   (2019-10-16)
Revision as of 14:35, 21 October 2019 by TitusWasHere (Talk | contribs)


Reverse primer for amplifying BBa_E0040

A reverse primer that is 31 base pairs long that can be used to amplify the GFP part BBa_E0040 by PCR.

A reverse primer that is 31 base pairs long that can be used to amplify the GFP part BBa_E0040 by PCR. It is used in conjunction with BBa_K2982006 or BBa_K2982006 in order to amplify a sequence that has an RBS with GFP (BBa_E0040). This is done for characterisation of BBa_J61124 by measuring its activity as an RBS.

This primer contains an XhoI restriction site and the last 21 nucleotides of the GFP sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None