Composite

Part:BBa_K3110054

Designed by: Amartya Pal   Group: iGEM19_IISER_Tirupati   (2019-10-16)
Revision as of 20:25, 18 October 2019 by Meghamariajacob (Talk | contribs)


J23117-YebF IL12-FLAG

YebF is a 13kDa protein that is known to be secreted into the extracellular medium by E. coli strains used in the lab. This particular protein was used with IL-12 in our design, in order to mediate the secretion of the cytokine from the bacteria. The part was used with constitutive promoter (J23100) but the results were inconclusive. Western blots were done with both the cell pellet and concentrated supernatant but the protein was not detected.Possible errors could be because of SOEing errors in the promoter and RBS regions.

The original YebF part can be found here: BBa_K1659003


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None