Part:BBa_K2924015

Promoter aldA from the Escherichia coli genome

The promoter aldA was published as an oleic acid (C18:1) sensitive promoter enabling for the measurement of free fatty acids present in microorganisms.

In Escherichia coli the growth on fatty acids requires many different proteins which are repressed by the transcriptional factor FadR.¹ The long chain acyl-CoA ester is an effector on the transcriptional factor FadR for the regulation of fatty acid metabolism.²

Fig.1: Regulation of the fatty acid metabolism by the transcriptional factor FadR. FadR recognizes its cognate binding site (white), thereby repressing transcription. Upon binding of an Acyl-CoA to FadR, the promoter region is freed, enabling gene expression.

After adding oleic acid (C18:1) many proteins showed an altered expression level and new proteins like AldA were synthesised. The promoter for aldA was also used for the production of green fluorescent protein (GFP).1 P_aldA was isolated from the Escherichia coli wild type genome. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]