Coding

Part:BBa_K2984032

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 14:37, 16 October 2019 by Chlamy Dima (Talk | contribs)


L1c-PsaD-ARS-PETase-3xHA-RBCS2

This part is composed of the PsaD promoter (BBa_K2984008), the ARS secretion signal (BBa_K2984000), the PETase enzyme (), a 3xHA tag (BBa_K2984015), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1769
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1308
    Illegal PstI site found at 1769
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 1032
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1769
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1769
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassisC. reinhardtii
proteinPETase
tag3xHA