Coding
cyt102A1 B

Part:BBa_K3038007:Design

Designed by: Anais CANTEAU   Group: iGEM19_Poitiers   (2019-10-16)
Revision as of 09:40, 16 October 2019 by Bvannier (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Cytochrome 102A1 BM3 Mutant S72Y V78A F87A _Cterm 6His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 81
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1225
    Illegal SapI.rc site found at 2248
    Illegal SapI.rc site found at 2848


Design Notes

RFC10 design

Optimized for GC content

6His tag added at the Cterm part (bp 3159 to 3176)

Source

sequence synthesis by IDT

References