Composite

Part:BBa_K3190601

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-16)
Revision as of 11:53, 16 October 2019 by IbenNikolajsen (Talk | contribs)


pGAL1-BI-I: BI-I CDS under inducible promoter

The coding sequence of BAX Inhibitor-I anti-toxin under the control of inducible promoter for the design of a kill switch system for a yeast wherein a BAX toxin is expressed under a constitutive promoter. This system ensures that the yeast survives only unless it is present in the medium with inducer.


Usage and Biology

UCopenhagen Killswitch.jpeg

Figure legend: Overview of the toxin/anti-toxin biosafety device.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 954
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 377
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
Parameters
None