Coding
INT8

Part:BBa_K3254008:Design

Designed by: Yeqing Zong   Group: iGEM19_GENAS_China   (2019-10-14)
Revision as of 13:15, 20 October 2019 by Zongyeqing (Talk | contribs) (→‎Source)

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Integrase Int8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 930
    Illegal AgeI site found at 1327
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was first reported in 2014. In this version, a PstI site and an EcoRI site were eleminated by synonymous mutations.


Source

The original organism is the Staphylococcus haemolyticus JCSC1435. The DNA was coloned from the source plasmid(pCis2+7+8+5, see reference).

References

Yang, L., et al., Permanent genetic memory with >1-byte capacity. Nat Methods, 2014. 11(12): p. 1261-6.