Coding

Part:BBa_K2980010

Designed by: Ji Gao   Group: iGEM19_Tsinghua   (2019-09-19)
Revision as of 11:44, 16 October 2019 by Billsfriend (Talk | contribs)

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Cry2-mCherry-xylE

Cry2-mCherry-xylE with Anderson promoter and LacI binding regulator region. Use this to prove the effect of phase separation on enzyme catalysis.


Usage and Biology

Exp/Ctrl ratio

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 497
    Illegal BglII site found at 956
    Illegal BamHI site found at 1435
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2767
    Illegal NgoMIV site found at 2939
    Illegal AgeI site found at 381
    Illegal AgeI site found at 1110
    Illegal AgeI site found at 3290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 733
    Illegal BsaI.rc site found at 142
    Illegal SapI.rc site found at 250


[edit]
Categories
Parameters
None