Coding

Part:BBa_K2942703

Designed by: Yihui Wang   Group: iGEM19_SYSU-Medicine   (2019-10-16)
Revision as of 12:25, 19 October 2019 by Lyt (Talk | contribs)


Nitroreductase

Nitroreductase is an enzyme that can catalyze the reduction of nitro aromatic compounds to aromatic amines. In our project we optimized the nitroreductase that we carried out human codon optimization on the original nitro reductase and added flag tag for labeling, which Increases the expression of protein as well as detection .

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 100
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 100
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 100
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 100
    Illegal AgeI site found at 655
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None