Composite

Part:BBa_K3239007

Designed by: Xingyu Zhou   Group: iGEM19_ShanghaiFLS_China   (2019-10-15)
Revision as of 05:35, 16 October 2019 by BrianSebZhou (Talk | contribs) (→‎Usage and Biology)


pAOX1-GFP

yEFGP3 reporter gene expressed by pAOX1.

Usage and Biology

This construct is the control used in our experiment. After homologous recombination into wild type P. pastoris, the EGFP expressed shall serve as an indicator of AOX1 expression levels. Note that the original pAOX1-AOX1 copy remains intact after the recombination.

Figure 1. Illustration of the pAOX1-GFP construct




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 935
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None