Part:BBa_K3239006

yEGFP3

A yeast- and FACS-optimized EGFP variant.

Usage and Biology

This yEGFP3 reporter gene is an EGFP variant optimized for yeast and FACS cell sorting.

Functional Parameters

yEGFP3 protein is reported to have a half-life of about 7h in Saccharomyces cerevisiae (Mateus et al., 2000), the budding yeast to which Pichia pastoris is very similar.

A single-base mutation is introduced for Type IIS compatibility. This single mutation does not affect the protein sequence.

Reference

Protein: https://www.ncbi.nlm.nih.gov/protein/82569482

Source: https://www.ncbi.nlm.nih.gov/nuccore/82569479

Mateus, C., & Avery, S. V. (2000). Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. Yeast, 16(14), 1313–1323. doi: 10.1002/1097-0061(200010)16:14<1313::aid-yea626>3.0.co;2-o

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]