Part:BBa_K3317051:Design
NhaS with an RFP reporter
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 849
Illegal AgeI site found at 961 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The promoter was modified for its constitutive production and its characterization, adding the BBa_J23119 part at the beginning of the sequence and replacing its UV-activated promoter from the original BBa_K1255002 part.
Source
The original sequence was described by Krulwich in 1994 as a hypothetical gene. However, this part has been optimized from the BBa_K1255002 part registered by the CIDEB-UANL_Mexico team in 2014.
References
CIDEB-UANL_Mexico. (2014). Capture module. Recuperado el 24 de abril del 2018 de http://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture. Huang, L. (2010). Growth kinetics of Escherichia coli O157:H7 in mechanically-tenderized beef. International Journal of Food Microbiology. No. 140, pp. 40–48. Krulwich, T.& Ivey, M. (1994) Sodium ion binding proteins. Recuperado el 24 de abril del 2018, de http://www.google.com.mx/patents/US5346815.