Part:BBa_K2905016
High pH Sensor
This part is to be used as a reporter gene that is active at high pH levels.
Measuring Expression/Fluorescence at different pH: Alma 2019
To determine if the part works as intended (expression at high pH, repression at pH less than 5.5), K2905016 (this part) or a control, BBa_J04450 were analyzed at different pH. Cells were grown to mid-log in LB, then pelleted and transferred to M9 minimal media with pH adjusted to ~4.5 or left untouched at 7, and then grown for about 14 hours shaking at 37C. Fluorescence was measured in a Mx3500.
As can be seen from the graph, there is a statistically significant difference between expression for the reporter when comparing acidic and neutral conditions. From this we would conclude that the reporter is working as intended, at least qualitatively - compare this data to the inverse part, BBa_K2762014.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1661
Illegal AgeI site found at 1773 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 332
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