Part:BBa_K3040115
pFadD_fadR binding site
pFadD promoter is one of the regulator in the enzymes of fatty acid biosynthesis in E. coli. It is composed of two fadR recognition sites, and one CRP binding site. The fatty acid metabolism system of E. coli is consist of many parts, fadD, fadL, fadR, fadA, etc. Interestingly, each enzyme in this family has its own different sequence of fadR recognition site in its promoter, which makes these promoter having different strength, yet can still be interchangeable for us to apply in our system. Based on this fact, we tried pFadD promoter to take place of the weak pFadBA promoter. With two fadR recognition sites native in pFadD, we assume a better result in the sensitivity and a lower leakage in our pFadD promoter. Yet, we further modified pFadD promoter by replacing its CRP binding site with a FadR recognition site of the sequence of pFadBA promoter.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 33
Illegal XbaI site found at 219 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 33
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 33
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 33
Illegal XbaI site found at 219 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 33
Illegal XbaI site found at 219 - 1000COMPATIBLE WITH RFC[1000]
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