Part:BBa_K3040111
pFadBA-Lac-1
We create a hybrid Fatty acid/acyl-CoA-regulated promoter by the combination of pLac with a modified promoter pFadBA. It is pointed out by the previous iGEM team, UPF CRG Barcelona_2018 iGEM, that the native promoter pFadBA has great leakage and is a weak promoter that isn’t ideal enough for our system. Therefore, to enhance the sensitivity and reduce the leakage of the promoter pFadBA for making it more suitable for our system, we improved our promoter based on the previous pfadBA DNA sequence from NTU_Taida 2012. pFL1 promoter has a fadR recognition site between its -10 and -35 region, and a promoter pLac behind -10 region. pFL1 promoter is designed to respond to changes of both fatty acid and an exogenous inducer, IPTG. The hybrid promoter pFL1 is fully activated only when both the fatty acids and IPTG are present.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 65
Illegal suffix found in sequence at 167 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 65
Illegal SpeI site found at 168
Illegal PstI site found at 182
Illegal NotI site found at 71
Illegal NotI site found at 175 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 65
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 65
Illegal suffix found in sequence at 168 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 65
Illegal XbaI site found at 80
Illegal SpeI site found at 168
Illegal PstI site found at 182 - 1000COMPATIBLE WITH RFC[1000]
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