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Coding
Part:BBa_K3040101
Designed by: Lai, Si-Ying Group: iGEM19_NTHU_Taiwan (2019-10-15)
Revision as of 15:41, 16 October 2019 by TALENTTIEN (Talk | contribs)
Lipase-Psychrobacter sp 7195.
Lipase A1 from Antarctic deep sea bacterium, Psychrobacter sp 7195., can catalyze the hydrolysis of triacylglycerols to glycerol and monoacylglycerols. We utilize the characteristic of lipase-having different catalytic activity under different temperature, to sense the temperature change. Lipase A1 reaches its catalytic activity peak at about 30℃, and decreases as temperature rise or drop. Furthermore, to purify our lipase A in an easier way, we design a His-tag sequence behind the lipase A.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 955
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Categories
Parameters
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