Composite
PBAD--TesA

Part:BBa_K3038006

Designed by: Anas CANTEAU   Group: iGEM19_Poitiers   (2019-10-15)
Revision as of 13:24, 19 October 2019 by Bvannier (Talk | contribs)


PBAD--TesA-CTer-6His--Mlut-Nter-Cmyc


Co-expression of the TesA and Mlut Enzymes under the control of the pBAD promoter


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 3474
    Illegal XhoI site found at 3717
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3608
    Illegal BsaI.rc site found at 3839
    Illegal SapI site found at 961
    Illegal SapI site found at 1964


[edit]
Categories
Parameters
None