Composite

Part:BBa_K3038005:Design

Designed by: Anas CANTEAU   Group: iGEM19_Poitiers   (2019-10-15)
Revision as of 13:20, 19 October 2019 by Bvannier (Talk | contribs)


PBAD/TesACTer6His/MlutNterCmyc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All the coding sequences have been designed with the RFC10 standard and optimized for the GC content.

A 6 his Tag has been added at the Cterm part of the TesA enzyme A <cmyc tag has been added at the Nterm part of the Mlut enzyme


Source

Cloning of the different part using the Biobrick design and classic cloning techniques

References