Tag

Part:BBa_K3187024

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)
Revision as of 15:46, 16 October 2019 by Lertu (Talk | contribs)


6x Histidine (His) Tag + TGA Stop Codon

The 6x His-Tag is used as a tag for detection and as an affinity tag in protein purification. An Imidazole gradient can later be used to elute the tagged protein from a column containing nickel. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None