Part:BBa_K3176020:Design

Specific aptamer for E. faecium

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We had to deal with the sequencing of our aptamer candidates and select the one with the highest affinity and specificity for E. faecium.

Source

BBa_K3176020 was made from a DNA library synthesized by Eurofins Genomics and selected using the Whole-cell SELEX method.

References

Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (2002). A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America, 99(12), 7877–7882. doi:10.1073/pnas.082243699

Falord, M., Mäder, U., Hiron, A., Débarbouillé, M., & Msadek, T. (2011). Investigation of the Staphylococcus aureus GraSR regulon reveals novel links to virulence, stress response and cell wall signal transduction pathways. PloS one, 6(7), e21323. doi:10.1371/journal.pone.0021323