DNA

Part:BBa_K3176020

Designed by: Tara Fournier   Group: iGEM19_Pasteur_Paris   (2019-10-15)
Revision as of 00:05, 22 October 2019 by Tarafrnr (Talk | contribs)


Specific aptamer for E. faecium

Aptamers are short single-stranded DNA or RNA oligonucleotides that can be selected to a wide variety of targets. They are similar to antibodies through their affinity and specificity and show a high potential as diagnostic and therapeutic tools, with many advantages when compared with antibodies. BBa_K3176020 is a DNA aptamer selected against E. faecium strain specifically from a random DNA library, using the Whole-Cell SELEX process. Because of its specificity and its affinity, it can be used to detect living E. faecium cells in any clinical samples when coupled to a fluorophore.

Usage and Biology

We tested this aptamer and its ability to detect E. faecium strains by measuring a change in electric potential across the nanotubes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None