Coding
Part:BBa_K2936007
Designed by: Weng Jiaqi Group: iGEM19_ZJUT-China (2019-10-09)
LacZ alpha
An alpha peptide chain encoded by a gene LacZ
Usage and Biology
Mechanism：
Form 1 The ABS350 of reaction solution of bacterial culture and ONPG
Fig1. The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to righ
From the figure 7 the color of the third group is enough obvious compared to the background， and at the same time, in order to balance the principle of economy, the ratio of bacterial culture (OD650=0.5) to TMB was designed to be 1:10.
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Categories
Parameters
| None |