Part:BBa_K2936007

LacZ alpha

An alpha peptide chain encoded by a gene LacZ

Usage and Biology

The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) )

Characterize

LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
Mechanism:

Experimental Results

Form 1 The absorbance of different reaction solution at the wavelength of350 nm

< img src="https://2019.igem.org/wiki/images/5/5a/T--ZJUT-China--igem-part07form.png" alt=""width="600px"> < img src="https://2019.igem.org/wiki/images/thumb/5/57/T--ZJUT-China--igem-part07reaction.jpg/800px-T--ZJUT-China--igem-part07reaction.jpg" alt=""width="600px">

Figure 1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]