Coding

Part:BBa_K2936007

Designed by: Weng Jiaqi   Group: iGEM19_ZJUT-China   (2019-10-09)
Revision as of 06:30, 15 October 2019 by Can can (Talk | contribs)


LacZ alpha

An alpha peptide chain encoded by a gene LacZ


Usage and Biology

The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) )

Characterize

LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
Mechanism: T--ZJUT-China--igem-part07jili.png

Experimental Results

Form 1 The absorbance of different reaction solution at the wavelength of350 nm
Figure1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None