Composite
Part:BBa_K3110032:Design
Designed by: Mrugank Dake Group: iGEM19_IISER_Tirupati (2019-10-14)
Revision as of 17:01, 21 October 2019 by Meghamariajacob (Talk | contribs)
Strong Promoter Medium RBS lldP+lldR+lldD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 329
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3651
Illegal AgeI site found at 465
Illegal AgeI site found at 928
Illegal AgeI site found at 1366
Illegal AgeI site found at 1525
Illegal AgeI site found at 1537
Illegal AgeI site found at 2283 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3088
Source
Strong promoter-Medium RBS-lldP flank and lldD flank-terminator were synthesized by IDT.
lldP+lldR+lldD were amplified from DH5alpha genome.
References
iGEM ETH zurich 2015
iGEM NUS Singapore 2016
Aguilera, L., Campos, E., Gimenez, R., Badia, J., Aguilar, J., & Baldoma, L. (2008). Dual Role of LldR in Regulation of the lldPRD Operon, Involved in L-Lactate Metabolism in Escherichia coli. Journal of Bacteriology, 190(8), 2997–3005. doi:10.1128/jb.02013-07