Regulatory
P-erm

Part:BBa_K3183000

Designed by: Natasha Cooke   Group: iGEM19_Oxford   (2019-09-20)
Revision as of 17:34, 14 October 2019 by Dschramm (Talk | contribs) (Characterisation)


Erythromycin Constitutive Promoter

The promoter is derived from the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAMβ1 isolated from Enterococcus faecalis (Swinfield et al., 1990). It was characterised in six strains of Lactobacillus reuteri and Lactococcus lactis spp. cremoris MG1363 by Lizier et al in 2010, see https://academic.oup.com/femsle/article/308/1/8/513999. It is a constitutive promoter which can be used in Lactobacillus reuteri DSM 20016.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




Characterisation

[edit]
Categories
Parameters
None