Coding

Part:BBa_K3279006:Design

Designed by: Liang Siwen   Group: iGEM19_CAU_China   (2019-10-14)
Revision as of 15:05, 15 October 2019 by SvenL (Talk | contribs) (→‎Source)


INP-N ( N-terminus of Ice-nucleation protein ) from Pseudomonas syringae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 333
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primary concern was BioBrick compatibility so that illegal sites in RFC[10] did not appear in the sequence. And the sequeces was finally designed to link with pET30a(+) and cenA gene/cex gene, so we added restriction enzyme cutting site NdeI at the 5' and EcoRV at the 3'.


Source

The sequence is originally from Pseudomonas syringae's genomic sequence and we did a codon optimization.

References