pMC_0_2_XX_PRO_placeholder
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Description
This part is one of the place holders published by iGEM Marburg 2019. These place holder parts can be used in assemblies to subsequently replace it with another part of the same type in a secondary Golden Gate assembly. These parts are very useful if a lot of constructs that only differ in one position are to be constructed. The advantages of using placeholder in high throughput assemblies are clear: A seven part assembly usually requires to screen multiple colonies before finding the right one, meaning that a lot of test digesting or sequencing is involved. This is feasible if one wants to construct only a few parts- for high throughput assemblies the cost and time does not scale well enough. A two part assembly however has an extremely high success rate, meaning that in most cases it is sufficient to just pick one colony to get correct sequencing results.
graphic showing the workflow for a placeholder based assembly
This two step assembly heavily cuts down the invested workload and the cost per sample. iGEM Marburg 2019 investigated these advantages on the example of a 20-piece large scale assembly LLLlink to results of placeholdersLLL.