Regulatory

Part:BBa_K3046003:Design

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-10-14)
Revision as of 17:24, 21 October 2019 by JMejlsted (Talk | contribs)

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PLEAPgpdA_1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 106
    Illegal AgeI site found at 35
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.


Source

This part is created on the basis of the upstream region of the gpdA ORF (Systematic ORF name: An16g01830) of many aspergillus genomes. It has been created using the proHMMoter software, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model


References

[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019.