Regulatory

Part:BBa_K3030012

Designed by: Xiaomeng Liu   Group: iGEM19_XJTLU-CHINA   (2019-09-13)
Revision as of 20:04, 21 October 2019 by Auniany (Talk | contribs)

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MS2-responsive ribozyme switch (Off to On) + PP7 coat protein

This part is a combination of MS2 ribozyme switch and PP7 coat protein to manipulate regulation process with another part automatically in the mammalian cells. We built up a three plasmids circuit which aimed to subsequently regulate expression level of gene(s) connected by 2A peptide with regulating protein (MS2/PP7).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

Kennedy, A. B., Vowles, J. V., d'Espaux, L., & Smolke, C. D. (2014). 'Protein-responsive ribozyme switches in eukaryotic cells', Nucleic acids research, 42(19), 12306–12321. [Online]. Available from: https:// www.ncbi.nlm.nih.g ov/pubmed/18331713? dopt=Abstract


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