Coding

Part:BBa_K3253003:Design

Designed by: HAOYU JIANG   Group: iGEM19_Nanjing   (2019-10-14)
Revision as of 14:14, 16 October 2019 by JustinJ (Talk | contribs)


Ghchi 6


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence contains multiple restriction site that there can be wrong restriction cutting. Therefore, we add extra restriction site in order to make it be successfully cut and transferred.


Source

This gene comes from the genomic sequence of red chicken-feet cotton, and it is synthesized by the IGEM official. The gene is often overexpressed in the plant in the conditions in which the plant receives mechanical damage.

References

Ma, Yin-Ping, et al. “Cloning and Functional Analysis of Chitinase Gene GbCHI from Sea-Island Cotton (Gossypium Barbadense).” Hereditas (Beijing), vol. 34, no. 2, 2012, pp. 240–247., doi:10.3724/sp.j.1005.2012.00240.