Composite

Part:BBa_K3110002

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 12:51, 14 October 2019 by Murgii (Talk | contribs)


lldP+lldD

lldP is L-Lactate Permease which permits the entry of L-Lactate inside the bacterial cell. lldD is L-Lactate dehydrogenase and functions by cleaving L-Lactate to Pyruvate. Using a combination of these genes, we intend to regulate the threshold of detection of Lactate by lldPRD regulatory region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2805
    Illegal AgeI site found at 411
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1471
    Illegal AgeI site found at 1483
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2242


[edit]
Categories
Parameters
None