Coding
Part:BBa_K3117023:Design
Designed by: Lena Schorr Group: iGEM19_FAU_Erlangen (2019-10-13)
Revision as of 22:52, 21 October 2019 by Lena Schorr (Talk | contribs)
scFv VL against GPA33 codon optimized for CHO expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was codon optimized for expression in mammalian cells.
Source
The sequence of this part origins from the patent (Bonvini et al., 2017)
References
[1]: Carreras-Sangrà, Nelson; Tomé-Amat, Jaime; García-Ortega, Lucía; Batt, Carl A.; Oñaderra, Mercedes; Martínez-del-Pozo, Alvaro et al. (2012): Production and characterization of a colon cancer-specific immunotoxin based on the fungal ribotoxin α-sarcin. In: Protein engineering, design & selection : PEDS 25 (8), S. 425–435. DOI: 10.1093/protein/gzs032.
[2]: Bonvini, E., Moore, P. A., Li, J. C., Johnson, L. S., & Shah, K. (2017). U.S. Patent Application No. 15/313,765.