Coding

Part:BBa_K3117015:Design

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-13)
Revision as of 23:39, 21 October 2019 by Lena Schorr (Talk | contribs)


SpyTag codon optimized for CHO expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization was performed for mammalian expression.


Source

SpyTag origins from genomic DNA.

References

1. Hatlem, Daniel; Trunk, Thomas; Linke, Dirk; Leo, Jack C. (2019): Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins. In: International journal of molecular sciences 20 (9). DOI: 10.3390/ijms20092129.