Composite

Part:BBa_K2922038:Design

Designed by: Man Yang   Group: iGEM19_XMU-China   (2019-10-13)
Revision as of 16:27, 13 October 2019 by HKbasic-lawc0mmittee (Talk | contribs) (→‎Design Notes)


The colicin-N operon under T7 promoter control with a gfasPurple chromoprotein reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1938
    Illegal NheI site found at 1961
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 598
    Illegal AgeI site found at 1602
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Each CDS are added an RBS (BBa_B0034) behind, and contain a terminator(BBa_B0010) in the end of operon`s sequence(BBa_B0010)

Source

This part constructs from BBa_K525998,BBa_K2922027,BBa_K2922028,BBa_K2922029,BBa_K1033917 by standard assembly.

References