Coding

Part:BBa_K3117006:Experience

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-13)
Revision as of 14:03, 21 October 2019 by EvaNeugebauer (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3117006

In our project the Igk leader was added to three proteins (BBa_K3117026, BBa_K3117030, BBa_K3117029) for the transport of the protein into the medium, after transfection into HEK 293T cells.

Bild Ernte Figure 1: Western blot of the harvest after transfection into HEK 293T cells with an anti-His-Tag antibody

In Fig. 1 the western blot of the harvest of HEK 293T cells can be seen. Those proteins were detected via their His-Tag. The presence of the proteins in the medium shows, that the Igk leader was able to direct all three proteins into the secretory way.

User Reviews

UNIQ781a6c47f27053ef-partinfo-00000000-QINU UNIQ781a6c47f27053ef-partinfo-00000001-QINU