Coding

Part:BBa_K3051001

Designed by: Megha Bawa   Group: iGEM19_Warwick   (2019-10-13)
Revision as of 20:54, 16 October 2019 by AleColosio (Talk | contribs)


Candida Antarctica Lipase A

Candida Antartica Lipase A (CALA) is a lipase extracted from the yeast Candida Antartica. It has been shown to work at very low temperatures. CALA has been shown to be very receptive to C8 p nitrophenol esters, compared to C4 and >C12 esters.

It has also been shown to be very sensitive to phenylmethylsulfonyl fluoride and high alcohol contents, causing a decrease in activity of as low as 50% compared to its control.[1]

It has a kDa of 49.3 and has been shown to react with p nitrophenol octanoate, to give p nitrophenol. Its molar extinction coefficient in water has been calculated to be 54570 M-1cm-1 (using ProtoPram ExPaSy tool). Its 3D structure has been calculated to be as follows:

T--Warwick--CALA_3D_structure.png

Sources 1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131601/


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None