Coding
hyg

Part:BBa_K2984012:Design

Designed by: Alejandro Gaston Castro   Group: iGEM19_Humboldt_Berlin   (2019-10-13)
Revision as of 11:47, 13 October 2019 by Alejandrcastro (Talk | contribs)

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Hygromycin B CDS; Selection for Transformation - Antibiotic resistance aphVII (hyg) B3-B4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 797
    Illegal NgoMIV site found at 905
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

Berthold, et al. (2002)


References

[1]Berthold, P., Schmitt, R., & Mages, W. (2002). An engineered Streptomyces hygroscopicus aph 7" gene mediates dominant resistance against hygromycin B in Chlamydomonas reinhardtii. Protist, 153(4), 401-412. doi:10.1078/14344610260450136