Plasmid_Backbone

Part:BBa_K2984003

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-12)
Revision as of 13:50, 17 October 2019 by Chlamy Dima (Talk | contribs)

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Level 1 a backbone with GoldenGate Fusionsite

This vector is a part of the Chlamy-HUB-Collection. E. coli backbone designed for Modular Cloning. Contains red fluorescent protein (RFP) flanked by BpiI recognition sites and ampicillin resistance for screening. The Level 1 plasmid “L1a” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (TGCC/GCAA). In comparison to the original pICH47732 backbone (Weber et al., 2011), the “L1a” plasmid has a decreased size of more than 2000 bases.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 693
    Illegal XhoI site found at 685
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal AgeI site found at 1499
    Illegal AgeI site found at 1611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1787
    Illegal BsaI.rc site found at 716
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Categories
Parameters
None