Plasmid_Backbone
L1b

Part:BBa_K2984005

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-12)
Revision as of 13:51, 17 October 2019 by Chlamy Dima (Talk | contribs)

Codon-Usage-Optimized Paromomycin Resistance For Use in Chlamydomonas reinhardtii

This vector is a part of the Chlamy-HUB-Collection. The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA). In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 298
    Illegal NotI site found at 122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 401
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None