Plasmid_Backbone

Part:BBa_K2984003

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-12)
Revision as of 11:41, 13 October 2019 by Sxvia (Talk | contribs)

E. coli<i/> backbone designed for Modular Cloning. Contains red fluorescent protein (RFP) flanked by BpiI recognition sites and ampicillin resistance for screening. The Level 1 plasmid “L1a” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (TGCC/GCAA). In comparison to the original pICH47732 backbone (Weber et al., 2011), the “L1a” plasmid has a decreased size of more than 2000 bases.

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