Regulatory
L7AeO

Part:BBa_K3179001:Design

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-13)
Revision as of 18:13, 19 October 2019 by Bigxushen (Talk | contribs) (→‎Source)


L7AeO(a optimized L7Ae which is a transcriptional level repressor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

L7Ae binds C/D boxes of sRNAs and guides RNA post-transcriptional modification; also binds KT-15 motif in 23S rRNA. L7Ae targets a specific sequence at the 5' end of the RNA, termed 2x-kturn. We use L7Ae to suppress another component 2xKturn-E1. Cooperating with another mRNA to play the role of miRNA sensor, identifying miRNA. We mutant one of its k-turn combine region, and reduce its cytotoxicity.


Source

We get this sequence from NCBI, and the sequence was synthesized from a biological company.

References