Coding

Part:BBa_K3140005:Design

Designed by: Fahad Ali   Group: iGEM19_Sydney_Australia   (2019-10-12)
Revision as of 10:44, 21 October 2019 by Fahad3li (Talk | contribs) (→‎Source)


VVD36-C73A - Truncated VIVID fluoroprotein derived from Neurospora crassa


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 434
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

The native sequence for VVD (Neurospora crassa) was obtained from NCBI (GenBank accession AF338412.1) and a coding sequence was generated by removing the first 36 amino acid residues and appending a start codon to the start of this truncated sequence. The cystine residue at position 73 (TGC) was then changed to alanine by changing the codon to GCC to yield VVD36-C73A.

References