Part:BBa_K2983060

RedStar2

RedStar2 is a derivative of the Red Fluorescent Protein from Discosoma sp. with improved features in terms of intrinsic brightness and speed of maturation [1]. The protein is tetrameric and contains 15 mutations compared to wild-type (Uniprot Q9U6Y8) : deltaR2, K5E, N6D, K9T, R17K, H41T, N42Q, V44A, V96I, F124L, A145P, M182K, P186Q, T202I, T217A.

This part is derived from coding sequence of RedStar2 used by Larroude et al. [2].

Usage and Biology

Applications are various: Fusions, promoter & secretion studies, FRET and other quantitative imaging techniques.

References

[1] Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M. A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast (2004) 21, 947-962.

[2] Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T. A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology. Microb Biotechnol (2019) 12, 1249– 1259.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]