Coding
ParoR

Part:BBa_K2984006

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-12)
Revision as of 21:39, 12 October 2019 by Chlamy Dima (Talk | contribs)


Codon-Usage-Optimized Paromomycin Resistance For Use in Chlamydomonas reinhardtii

E.coli backbone designed for Modular Cloning. Contains red fluorescent protein (RFP) flanked by BpiI recognition sites and ampicillin resistance for screening.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 298
    Illegal NotI site found at 122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 401
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None