Plasmid_Backbone
L1b
Part:BBa_K2984005
Designed by: Dimitri Schumacher Group: iGEM19_Humboldt_Berlin (2019-10-12)
Revision as of 11:01, 13 October 2019 by Alejandrcastro (Talk | contribs)
The Level 1 plasmid “L1a” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA). In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases.
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