Regulatory
Pveg

Part:BBa_K143012:Design

Designed by: James Chappell   Group: iGEM08_Imperial_College   (2008-09-11)
Revision as of 11:25, 18 September 2008 by Chris (Talk | contribs) (→‎Source)

Promoter veg a constitutive promoter for B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The biobrick part was designed to include a single binding site for the B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.


Source

The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. The sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.

References

<biblio>

  1. 1 pmid=6181373
  2. 2 pmid=6820069
  3. 3 pmid=14651647
  4. 4 pmid=17962296

</biblio>