Part:BBa_K1033910
fwYellow, yellow chromoprotein
This chromoprotein (also known as FezziwigYFP) naturally exhibits strong color when expressed. Compared to many other chromoproteins, such as amilCP (BBa_K592009), amilGFP (BBa_K592010), spisPink (BBa_K1033932), asPink (BBa_K1033933) and aeBlue (BBa_K864401), the color development is slower. The color is readily observed in both LB or on agar plates after 24-64 hours of incubation.
Usage and Biology
This part is useful as a reporter.
iGEM13_Uppsala: Expression of chromoproteins. The images above show E coli constitutively expressing meffRed BBa_K1033922, aeBlue BBa_K1033929, tsPurple BBa_K1033905, asPink (asCP) BBa_K1033927, fwYellow BBa_K1033910, meffBlue BBa_K1033902, meffRed BBa_K1033922 and scOrange BBa_K1033913.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 109
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 486
Characterization: NWU-China 2019
The NWU-China team overexpressed fwYellow protein in E. coli and extracted and purified. The maximum absorbance was measured in a 96-well plate using a microplate reader (Synergy 2).
//collections/chromoprotein/uppsala
//function/reporter/color
//function/reporter/pigment
abs | 523nm |
color | Yellow |
emission | 510 nm |
excitation | 508 nm |